RNAmediator.GenerateBigWig.generate_bws

RNAmediator.GenerateBigWig.generate_bws(goi, filelist, chrom, gs, ge, gstrand, ulim, cutoff, border, outdir, padding, chromstr, queue=None, configurer=None, level=None)

Generate BigWig entries

Parameters:
  • filelist (list) – List of files to work on

  • chrom (str) – Chromosome

  • gs (int) – Start coordinate of gene

  • ge (int) – End coordinate of gene

  • gstrand (str) – Strand of gene

  • ulim (int) – Stretch of nucleotides used during plfold run (-u option)

  • cutoff (float) – Cutoff for the definition of pairedness, if set to < 1 it will select only constraint regions with mean raw (unconstrained) probability of being unpaired <= cutoff for further processing(default: 1.0)

  • border (float) – Cutoff for the minimum change between unconstrained and constraint structure, regions below this cutoff will not be further evaluated.

  • outdir (str) – Directory to write to

  • padding (int) – Padding around constraint that will be excluded from report, default is 1, so directly overlapping effects will be ignored

  • queue (Multiprocessing.Queue, optional) – Queue used for logging process

  • configurer (Function, optional) – Function to configure logging

  • level (str, optional) – Loglevel