RNAmediator.GenerateBigWig.generate_bws
- RNAmediator.GenerateBigWig.generate_bws(goi, filelist, chrom, gs, ge, gstrand, ulim, cutoff, border, outdir, padding, chromstr, queue=None, configurer=None, level=None)
Generate BigWig entries
- Parameters:
filelist (list) – List of files to work on
chrom (str) – Chromosome
gs (int) – Start coordinate of gene
ge (int) – End coordinate of gene
gstrand (str) – Strand of gene
ulim (int) – Stretch of nucleotides used during plfold run (-u option)
cutoff (float) – Cutoff for the definition of pairedness, if set to < 1 it will select only constraint regions with mean raw (unconstrained) probability of being unpaired <= cutoff for further processing(default: 1.0)
border (float) – Cutoff for the minimum change between unconstrained and constraint structure, regions below this cutoff will not be further evaluated.
outdir (str) – Directory to write to
padding (int) – Padding around constraint that will be excluded from report, default is 1, so directly overlapping effects will be ignored
queue (Multiprocessing.Queue, optional) – Queue used for logging process
configurer (Function, optional) – Function to configure logging
level (str, optional) – Loglevel